کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795699 | 1052592 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Epigallocatechin gallate counteracts oxidative stress in docosahexaenoxic acid-treated myocytes
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کلمات کلیدی
MMPFluo-3 AMND3MFN2PUFAsFluo-3 acetoxymethyl esterDrp1MnSODFCCPAFUEpigallocatechin-3-gallateDCFH-DAETsnDNAGAPDHEGCGOXPHOSDMEMCOXFBS2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetateMitochondrial DNA - DNA میتوکندریاNuclear DNA - DNA هسته ایopa1 - grandpa1Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccooptic atrophy 1 - آتروفی اپتیکی 1docosahexaenoic acid - اسید داکوزاگزوائونیکPolyunsaturated fatty acids - اسید چرب اشباع نشدهDHA - دوکوساهگزائنوئیک اسیدmtDNA - دیانای میتوکندریاییelectron transport chain - زنجیره انتقال الکترونfetal bovine serum - سرم جنین گاوmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزCitrate synthase - سیترات سیتواستاتOxidative phosphorylation - فسفوریلاسیون اکسیداتیوlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH mitofusin 2 - میتوفوسین 2ETc - و غیرهarbitrary fluorescence units - واحدهای فلورسانس دلخواهMitochondrial membrane potential - پتانسیل غشای میتوکندریmitochondrial fission 1 protein - پروتئین تقسیم میتوکندری 1dynamin-related protein 1 - پروتئین مرتبط با دینام 1carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazoneglyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Skeletal muscle is a key organ of mammalian energy metabolism, and its mitochondria are multifunction organelles that are targets of dietary bioactive compounds. The goal of this work was to examine the regulation of mitochondrial dynamics, functionality and cell energy parameters using docosahexaenoic acid (DHA), epigallocatechin gallate (EGCG) and a combination of both in L6 myocytes. Compounds (at 25 μM) were incubated for 4 h. Cells cultured with DHA displayed less oxygen consumption with higher ADP/ATP ratio levels concomitant with downregulation of Cox and Ant1 gene expression. The disruption of energetic homeostasis by DHA, increases intracellular reactive oxygen species (ROS) levels and decreases mitochondrial membrane potential. The defence mechanism to counteract the excess of ROS production was by the upregulation of Ucp2, Ucp3 and MnSod gene expression. Moreover myocytes cultured with DHA had a higher mitochondrial mass with a higher proportion of large and elongated mitochondria, whereas the fission genes Drp1 and Fiss1 and the fusion gene Mfn2 were downregulated. In myocytes co-incubated with DHA and EGCG, ROS levels and the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) ratio were similar to untreated myocytes and the decrease of oxygen consumption, higher mitochondrial mass and the overexpression of Ucp2 and Ucp3 genes were similar to the DHA-treated cells with also a higher amount of mitochondrial deoxyribonucleic acid (DNA), and reduced Drp1 and Fiss1 gene expression levels. In conclusion the addition of EGCG to DHA returned the cells to the control conditions in terms of mitochondrial morphology, energy and redox status, which were unbalanced in the DHA-treated myocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 6, June 2014, Pages 783-791
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 6, June 2014, Pages 783-791
نویسندگان
Ester Casanova, Laura Baselga-Escudero, Aleix Ribas-Latre, Anna Arola-Arnal, Cinta Bladé, LluÃs Arola, M. Josepa Salvadó,