کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795795 | 1052596 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
On the analysis of non-photochemical chlorophyll fluorescence quenching curves
ترجمه فارسی عنوان
بر روی تجزیه و تحلیل منوهای خنک کننده فلورسانس کلروفیل غیر فتوشیمیایی
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کلمات کلیدی
NPQPS IIPS I - PS منEnergy dissipation - اتلاف انرژیKinetic analysis - تجزیه سینتیکsupporting information - حمایت از اطلاعاتNon-photochemical quenching - خنک سازی غیر فتوشیمیاییFluorescence quenching - خنک شدن فلورسنتEnergy-dependent quenching - رطوبت وابسته به انرژیphotosystem I - عکس سیستم منPhotoprotection - عکسبرداریPhotosynthesis - فتوسنتزPhotosystem II - فتوسیستم 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Non-photochemical quenching (NPQ) protects photosynthetic organisms against photodamage by high light. One of the key measuring parameters for characterizing NPQ is the high-light induced decrease in chlorophyll fluorescence. The originally measured data are maximal fluorescence (Fmâ²) signals as a function of actinic illumination time (Fmâ²(t)). Usually these original data are converted into the so-called Stern-Volmer quenching function, NPQSV(t), which is then analyzed and interpreted in terms of various NPQ mechanisms and kinetics. However, the interpretation of this analysis essentially depends on the assumption that NPQ follows indeed a Stern-Volmer relationship. Here, we question this commonly assumed relationship, which surprisingly has never been proven. We demonstrate by simulation of quenching data that particularly the conversion of time-dependent quenching curves like Fmâ²(t) into NPQSV(t) is (mathematically) not “innocent” in terms of its effects. It distorts the kinetic quenching information contained in the originally measured function Fmâ²(t), leading to a severe (often sigmoidal) distortion of the time-dependence of quenching and has negative impact on the ability to uncover the underlying quenching mechanisms and their contribution to the quenching kinetics. We conclude that the commonly applied analysis of time-dependent NPQ in NPQSV(t) space should be reconsidered. First, there exists no sound theoretical basis for this common practice. Second, there occurs no loss of information whatsoever when analyzing and interpreting the originally measured Fmâ²(t) data directly. Consequently, the analysis of Fmâ²(t) data has a much higher potential to provide correct mechanistic answers when trying to correlate quenching data with other biochemical information related to quenching.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1827, Issue 6, June 2013, Pages 786-792
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1827, Issue 6, June 2013, Pages 786-792
نویسندگان
Alfred R. Holzwarth, Dagmar Lenk, Peter Jahns,