کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10796128 | 1052694 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In intact leaves, the maximum fluorescence level (FM) is independent of the redox state of the plastoquinone pool: A DCMU-inhibition study
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DCMUΨ0φPoTR0/RCET0/RCLHCIIChlPSIIPSI3-(3′,4′-dichlorophenyl)-1,1-dimethylurea - 3- (3 '، 4'-dichlorophenyl) -1،1-dimethylureaABS/RC - ABS / RCQA and QB - QA و QBmaximum fluorescence - حداکثر فلورسنسlight-emitting diode - دیود نورافشانLED - رهبریChlorophyll - سبزینه یا کلروفیلCross section - سطح مقطعphotosystem I - عکس سیستم منPhotosystem II - فتوسیستم 2reaction center - مرکز واکنشPlastocyanin - پلاستوسیانینplastoquinone - پلواستیکینونChlorophyll a fluorescence - کلروفیل یک فلورسانس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effects of DCMU (3-(3â²,4â²-dichlorophenyl)-1,1-dimethylurea) on the fluorescence induction transient (OJIP) in higher plants were re-investigated. We found that the initial (F0) and maximum (FM) fluorescence levels of DCMU-treated leaves do not change relative to controls when the treatment is done in complete darkness and DCMU is allowed to diffuse slowly into the leaves either by submersion or by application via the stem. Simultaneous 820 nm transmission measurements (a measure of electron flow through Photosystem I) showed that in the DCMU-treated samples, the plastoquinone pool remained oxidized during the light pulses whereas in uninhibited leaves, the FM level coincided with a fully reduced electron transport chain. The identical FM values with and without DCMU indicate that in intact leaves, the FM value is independent of the redox state of the plastoquinone pool. We also show that (i) the generally observed F0 increase is probably due to the presence of (even very weak) light during the DCMU treatment, (ii) vacuum infiltration of leaf discs leads to a drastic decrease of the fluorescence yield, and in DCMU-treated samples, the FM decreases to the I-level of their control (leaves vacuum infiltrated with 1% ethanol), (iii) and in thylakoid membranes, the addition of DCMU lowers the FM relative to that of a control sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1708, Issue 2, 30 June 2005, Pages 275-282
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1708, Issue 2, 30 June 2005, Pages 275-282
نویسندگان
Szilvia Z. Tóth, Gert Schansker, Reto J. Strasser,