Selective antimicrobial activity and mode of action of adepantins, glycine-rich peptide antibiotics based on anuran antimicrobial peptide sequences

► Algorithm was trained on anuran AMPs with known haemolytic/antimicrobial activities. ► We used it to design AMPs highly selective for Gram-negative bacteria. ► Monomeric and dimeric analogues were prepared to study the mode of action. ► The peptides act on the bacterial membrane in a highly selective manner.