Non-reducing trisaccharide fatty acid monoesters: Novel detergents in membrane biochemistry

► The new surfactants, regioisomerically pure, cover CMCs from μM to mM. ► They solubilize membrane proteins independently of their lipidic environment. ► They keep the activity of solubilized proteins better than classical detergents. ► They are fully compatible with chromatographic protein purification methods. ► Large unilamellar (proteo)liposomes are produced by their enzymatic hydrolysis.