Keywords: Escherichia coli; Codon optimization; Kynurenine aminotransferase; Ni-NTA; HPLC; Crystallization
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Keywords: 2Nal; 3-(2-naphthy)-alanine; AFWK; pheylalanine/tryptophan/lysine triple auxotrophic E. coli; DHB; 2,5-dihydroxybenzoic acid; DHF; dihydrofolate; DHFR; dihydrofolate reductase; Glu; glutamic acid; LB; Lria-Broth; Lys; lysine; MALDI-TOF MS; matrix-assisted
Keywords: Biacore; ProteOn; Octet; MX96; Antibody-antigen interactions; Optical biosensor; mAb; monoclonal antibody; SPR; surface plasmon resonance; BLI; Bio-Layer Interferometry; CFM; continuous flow microfluidics; PCSK9; proprotein convertase substilisin kexin ty
Keywords: Abz; 2-aminobenzoyl; BNA; β-naphthylamide; CD; circular dichroism; DLS; dynamic light scattering; DSC; differential scanning calorimetry; DTT; dithiothreitol; DTNB; 5,5'-dithiobis(2-nitrobenzoic acid); EDTA; ethylenediaminetetraacetic acid; EDDnp; N-(2,4
Cloning, overexpression, and purification of a gene of unknown function of prophage loci from 'Candidatus Liberibacter asiaticus,' the destructive bacterial pathogen of huanglongbing disease in citrus plants
Keywords: Candidatus Liberibacter asiaticus'; Huanglongbing disease; Blotchy mottle; Lime; Type-D; Prophage; CLas; 'Candidatus Liberibacter asiaticus'; E. coli; Escherichia coli; A. tumefaciens; Agrobacterium tumefaciens; A. thaliana; Arabidopsis thaliana; C.
Evaluation of LIPS (luciferase immunoprecipitation system) for serodiagnosis of Toxoplasmosis
Keywords: Luciferase immunoprecipitation system; Nanoluciferase Toxoplasma gondii; Recombinant proteins; Anti-Toxoplasma human immunoglobulin; Serodiagnosis; LIPS; luciferase immunoprecipitation system; Nluc; nanoluciferase; TOXM; Toxoplasma human immunoglobulin; E
The Shewanella woodyi galactokinase pool phosphorylates glucose at the 6-position
Keywords: Galactokinase; Hexokinase; GHMP family; Shewanella woodyi; ADP; adenosine diphosphate; ATP; adenosine triphosphate; EDTA; ethylenediamine tetraacetic acid; G6P; glucose-6-phosphate; G6PDH; glucose-6-phosphate dehydrogenase; GHMP; galactokinase, homoserine
Biophysical characterization and stabilization of detergent-solubilized lipoprotein N-acyl transferase from P. aeruginosa and E. coli
Keywords: ε; molar extinction coefficient (Mâ1â¯cmâ1); EDTA; ethylenediaminetetraacetic acid; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HRP; horseradish peroxidase; IMAC; immobilized-metal affinity chromatography; IPTG; isopropyl β-d-1-thiog
Insights into the functional properties of the marneral oxidase CYP71A16 from Arabidopsis thaliana
Keywords: P450; cytochrome P450 monooxygenase; CPR; cytochrome P450 reductase; LB; lysogeny broth (medium); TB; terrific broth (medium); IPTG; isopropyl-β-d-thiogalactopyranoside; GC/MS; gas chromatography coupled with mass spectrometry; HPLC; high performance liq
Highly stable single-strand-specific 3â²-nuclease/nucleotidase from Legionella pneumophila
Keywords: 3â²-AMP; adenosine 3â²âmonophosphate; 5â²-AMP; adenosine 5â²âmonophosphate; CD; circular dichroism; DTT; 1,4âdithiotreitol; EDTA; ethylenediaminetetraacetic acid; IEF; isoelectric focusing; LCV; Legionella-containing vacuole; Lp; Legionella pneu
Expression of Cry1Ac toxin-binding region in Plutella xyllostella cadherin-like receptor and studying their interaction mode by molecular docking and site-directed mutagenesis
Keywords: BSA; bovine serum albumin; cDNA; complementary DNA; ELISA; enzyme-linked immunosorbent assay; HRP; horse radish peroxidase; IPTG; isopropyl-beta-d-thiogalactopyranoside; MPBS; PBS containing skim milk; Ni-NTA; nickel-nitrilotriacetic acid; OD; optical den
Optimization of expression and purification of human mortalin (Hsp70): Folding/unfolding analysis
Keywords: Hsp; heat-shock protein; CD; circular dichroism; ANS; 1-anilinonaphthalene-8-sulfonate; Ni-NTA; Nickel-nitrilotriacetic acid; Mortalin; Intrinsic fluorescence; Chaperone; CD;
Microbial F-type lectin domains with affinity for blood group antigens
Keywords: F-type lectin domain; Fucose; Blood group antigens; Myxococcus hansupus FLD; Cyanobium sp. FLD; Leucothrix mucor FLD; FLD; F-type Lectin Domain; CyFkbMFLD; Cyanobium sp. PCC 7001 FkbMFLD; CyFLD; Cyanobium sp. PCC 7001 FLD; MhFLD1; Myxococcus hansupus FLD1
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis
Keywords: Crystal structure; Toxin-antitoxin; tRNA endonuclease; Structure-function; Substrate specificity; AIDS; acquired immune deficiency syndrome; BL17U; beamline 17U; M. tb; Mycobacterium tuberculosis; PSP; PreScission protease; DTT; dithiothreitol; PMSF; phe
Biophysical characterization of soluble Pseudomonas syringae ice nucleation protein InaZ fragments
Keywords: AUC; analytical ultracentrifugation; AFP; anti-freeze protein; CD; circular dichroism; FPLC; fast protein liquid chromatography; INP; ice nucleation protein; IR; infrared; LeIBP; Leucosporidium ice-binding protein; Ni-NTA; Ni2+-nitrilotriacetic acid; NMR;
Research articleUse of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes
Keywords: Biolistic chloroplast transformation; CP47 His-tag; Photosystem II; Transplastomic tobacco plants; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; MES; 2-(N-morpholino)ethanesulfonic acid; Ni-NTA; nickel-nitrilotriacetic acid;
Stable immobilisation of His-tagged proteins on BLI biosensor surface using cobalt
Keywords: His-tagged protein; Ni-NTA; Biolayer interferometry; Avidin-biotin; Norovirus; Label-free detection;
Structure, biochemical and kinetic properties of recombinant Pst2p from Saccharomyces cerevisiae, a FMN-dependent NAD(P)H:quinone oxidoreductase
Keywords: 1,4-BQ; 1,4-benzoquinone; CoQ1; Coenzyme Q1; DQ; duroquinone; S. cerevisiae; Saccharomyces cerevisiae; E. coli; Escherichia coli; EtOH; ethanol; HQ; hydroquinone; Lot6; low temperature-responsive 6; MQ; menadione; Ni-NTA; nickel-nitrilotriacetic acid; NQO
Cloning, characterization and subcellular localization of Nuclear LIM interactor interacting factor gene from Leishmania donovani
Keywords: VL; Visceral Leishmaniasis; NLI; Nuclear LIM interacting-interactor like protein; Ldb; LIM domain binding protein; LdNLI; Leishmania donovani Nuclear LIM interacting-interactor like protein; CDRI; Central Drug Research Institute; ORF; Open reading frame;
Molecular architecture of an N-formyltransferase from Salmonella enterica O60
Keywords: CHES; 2-(cyclohexylaminoethanesulfonic acid); dTDP; thymidine diphosphate; Fuc3N; 3-amino-3,6-dideoxygalactose; Fuc3NFo; 3-formamido-3,6-dideoxygalactose; HEPPS; N-2-hydroxyethylpiperazine-Nâ²-3-propanesulfonic acid; HPLC; high-performance liquid chromat
Highly potential compatible solute from halotolerant Bacillus subtilis MA04: Its functional and molecular characterization
Keywords: GbsA; betaine aldehyde dehydrogenase; GbsB; betaine alcohol dehydrogenase; DEAE; diethylaminoethyl; X-gal; 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside; Ni-NTA; nickel-nitriloacetic acid column; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel
The unusual substrate specificity of a virulence associated serine hydrolase from the highly toxic bacterium, Francisella tularensis
Keywords: DSF; differential scanning fluorimetry; FTT0941c; virulence associated serine hydrolase from Francisella tularensis; HSL; hormone sensitive lipase; KEGG; Kyoto Encyclopedia of Genes and Genomes; LB; Luria-Bertani broth; MWCO; molecular weight cut-off; Ni-
Molecular cloning and in silico studies of physiologically significant trehalase from Drosophila melanogaster
Keywords: DmTre; D. melanogaster trehalase; dNTP; deoxy nucleotide triphosphate; DTT; dithiothreitol; EtBr; ethidium bromide; h; hour; HRP; horse radish peroxide; IPTG; isopropyl-β-d-thiogalactopyranoside; LB; Luria Bertani; min; minutes; Ni-NTA; nickel-nitrilotri
Structural insights into the mechanism of Escherichia coli YmdB: A 2â²-O-acetyl-ADP-ribose deacetylase
Keywords: ADPr; ADP-ribose; OAADPr; O-acetyl-ADP-ribose; NAD; nicotinamide adenine dinucleotide; ITC; isothermal titration calorimetry; PCR; polymerase chain reaction; LB; Luria-Bertani; IPTG; isopropyl β-d-1-thiogalactopyranoside; Ni-NTA; Ni-nitrilo acetic acid
Cloning, expression analysis, and functional characterization of two secretory phospholipases A2 in durum wheat (Triticum durum Desf.)
Keywords: BEL; bromoenol lactone; DAS; days after sowing; DTT; dithiothreitol; DWL-PLA2; PLA2 activity in durum wheat leaves; ER; endoplasmic reticulum; E.U.; enzymatic units; FFA; free fatty acid; GFP; green fluorescent protein; GSH; reduced glutathione; GSSG; oxi
Development and characterization of an αvβ6-specific diabody and a disulfide-stabilized αvβ6-specific cys-diabody
Keywords: NOTA-maleimide; 1,4,7-triazacyclononane-1,4,7-triacetic acid maleimide; Ni-NTA; nickel nitrilotriacetic acid; RCY; radiochemical yield; RCP; radiochemical purity; mAbs; monoclonal antibodies; immuno-PET; immuno-positron emission tomography; kDa; kilodalto
G protein-membrane interactions I: Gαi1 myristoyl and palmitoyl modifications in protein-lipid interactions and its implications in membrane microdomain localization
Keywords: AcNPV; Autographa californica nuclear polyhedrosis virus; AMF; 30 μM AlCl3, 50 mM MgCl2 and 10 mM NaF; Biotin-HPDP; N-[6-(biotinamido)hexyl]-3â²-(2â²-pyridyldithio) propionamide; C12E10; polyoxyethylene 10-lauryl ether; CHO; cholesterol; GPCR; G pro
Production of fully assembled and active Aquifex aeolicus F1FO ATP synthase in Escherichia coli
Keywords: IPTG; isopropyl-β-thiogalactopyranoside; DM; n-decyl-β-d-maltoside; DDM; n-dodecyl-β-d-maltoside; α-PCC; trans-4-(trans-4â²-propylcyclohexyl)cyclohexyl-α-d-maltoside; BN-PAGE; blue native polyacrylamide gel electrophoresis; IMAC; immobilized-metal a
Identification of a novel ATPase activity in 14-3-3 proteins - Evidence from enzyme kinetics, structure guided modeling and mutagenesis studies
Keywords: WT; wild-type; Ni-NTA; nickel-nitriloacetic acid; MALDI; matrix assisted laser desorption ionization; TOF; tandem time of flight; PDB; protein data bank; ATP-γ-S; adenosine 5â²-(3-thiotriphosphate); AMP-PCP; adenylylmethylenediphosphonate; HEPES; 4-(2-h
Novel membrane-associated prostaglandin E synthase-2 from crustacean arthropods
Keywords: AA; arachidonic acid; GSH; glutathione; GST; glutathione S-transferase; COX; cyclooxygenase; HHT; 12(S)-hydroxy-5,8,10(Z,E,E)-heptadecatrienoic acid; HPLC; high performance liquid chromatography; MDA; malondialdehyde; Ni-NTA; nickel nitrilotriacetic acid;
Subcutaneous inverse vaccination with PLGA particles loaded with a MOG peptide and IL-10 decreases the severity of experimental autoimmune encephalomyelitis
Keywords: BCA; bicinchoninic acid; CHOs; Chinese hamster ovary cells; CNS; central nervous system; CTL; cytotoxic T lymphocytes; DCM; dichloromethane; DLS; dynamic light scattering; EAE; experimental autoimmune encephalomyelitis; FDA; food and drug administration;
Crystal structure of HlyU, the hemolysin gene transcription activator, from Vibrio cholerae N16961 and functional implications
Keywords: wHTH; winged helix-turn-helix; CT; cholera toxin; IPTG; Isopropyl β-d-1-thiogalactopyranoside; Ni-NTA; nickel-nitrilotriacetic acid; PEG; polyethylene glycol; TCEP; (tris(2-carboxyethyl)phosphine); RMSD; root mean square deviation; SDM; structural di
Anti-amyloidogenic property of human gastrokine 1
Keywords: Gastrokine 1; Gastric cancer; BRICHOS domain; Aβ peptide; APP; Amyloidogenesis; Aβ(1-40); amyloid-beta peptide 1-40; GKN1; gastrokine 1; flGKN1; full length GKN1; rGKN1; recombinant GKN1; proSP-C; prosurfactant protein C; Ni-NTA; nickel-nitrilotriacet
A novel bispecific antibody targeting tumor necrosis factor α and ED-B fibronectin effectively inhibits the progression of established collagen-induce arthritis
Keywords: RA; rheumatoid arthritis; CIA; collagen-induced arthritis; HSA; human serum albumin; HRP; horseradish peroxidase; TNF; tumor necrosis factor; TNF-scFv; anti-TNF-α scFv; CD3-scFv; anti-CD3 scFv; IMAC; immobilized-metal affinity chromatography; MTT; 3-(4,5
Interactions involved in grasping and locking of the inhibitory peptide IF1 by mitochondrial ATP synthase
Keywords: F0F1-ATPase; ATP synthase complex (EC 3.6.3.14); F1-ATPase; isolated extrinsic part of ATP synthase; IF1; endogenous inhibitory peptide of mitochondrial ATP synthase; yIF1; IF1 from yeast; SMP; submitochondrial particle; pmf; protonmotive force; Tris; tri
Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target
Keywords: Ebselen; ROS levels; Mitochondrial membrane potential; Glutamate dehydrogenase; Histone clipping; Yeast sporulation; CypA; Cyclophilin A; DCFH-DA; 2,7-dichlorodihydrofluorescein diacetate; FACS; florescence activated cell sorting; GDH; glutamate dehydroge
A role for glutamate-333 of Saccharomyces cerevisiae cystathionine γ-lyase as a determinant of specificity
Keywords: AAT; aspartate aminotransferase; ACCS; 1-aminocyclopropane-1-carboxylate synthase; AMPSO; N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid; bicine; 2-(Bis(2-hydroxyethyl)amino)acetic acid; CAPS; 3-(Cyclohexylamino)-1-propanesulfonic a
Structural and functional effects of circular permutation on firefly luciferase: In vitro assay of caspase 3/7
Keywords: DEVDG; Asp-Glu-Val-Asp-Gly; Ppy; Photinus pyralis; ATP; adenosine triphosphate; ANS; 1-anilino-8-naphthalene sulfonate; CD; circular dichroism; Ni-NTA; nickel nitrilotriacetic acid; Trp; tryptophan; Pro; prolin; CP-1; circular permuted form 1; CP-2; circu
Integral role of the Iâ²-helix in the function of the “inactive” C-terminal domain of catalase-peroxidase (KatG)
Keywords: KatG; catalase-peroxidase; wtKatG; wild-type KatG; KatGN; separately expressed and isolated KatG N-terminal domain; KatGC; separately expressed and isolated KatG C-terminal domain; LL1; Large Loop 1; LL2; Large Loop 2; APx; ascorbate peroxidase; CcP; cy
Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions
Keywords: OAS; O-acetylserine; AVG; aminoethoxyvinylglycine; CBL; cystathionine β-lyase; CGL; cystathionine γ-lyase; CGS; cystathionine γ-synthase; Cth; cystathionine; Hcys; homocysteine; HO-HxoDH; d-2-Hydroxyisocaproate dehydrogenase; IPTG; Isopropyl-β-d-thiog
Oligomerization of rice granule-bound starch synthase 1 modulates its activity regulation
Keywords: OsGBSS1; Oryza sativa granule-bound starch synthase 1; ADPG; adenosine diphosphate glucose; AGPase; ADP-glucose pyrophosphorylase; CAPS; 3-(cyclohexylamino)-1-propanesulfonic acid; DBE; starch debranching enzyme; DPA; days past anthesis; DTNB; 5,5â²-dith
Expression and characterization of catalytic domain of Plasmodium falciparum subtilisin-like protease 3
Keywords: PMSF; phenylmethylsulfonyl fluoride; Ni-NTA; nickel-nitrilotriacetic acid; MALDI-MS; matrix-assisted laser desorption ionization; MS; mass spectrometry; Plasmodium falciparum; Serine protease; Subtilisin-like protease; Erythrocytic schizogony;
The structural basis for the narrow substrate specificity of an acetyl esterase from Thermotoga maritima
Keywords: 7-ACA; 7-aminocephalosporanic acid; BTB; bromothymol blue; CE7; carbohydrate esterase family 7; DSF; differential scanning fluorimetry; LB; Luria-Bertani broth; MWCO; molecular weight cut-off; Ni-NTA; nickel-nitrilotriacetic acid; NPA; 4-nitrophenol ace
Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins
Keywords: Ribotoxin; Cytotoxic protein; Ribosome; Ribonucleases; α-Sarcin; HtA; hirsutellin A; SRL; sarcin-ricin loop; IPTG; isopropyl α-d-thiogalactopyranoside; Tris; tris(hydroxymethyl)aminomethane; Mops; 3-[N-morpholino]propanesulfonic acid; Ni-NTA; Ni2+ nit
Subcellular localization of adenylate kinases in Plasmodium falciparum
Keywords: aa; amino acid; AK; adenylate kinase; AKLP1; adenylate kinase-like protein 1; AKLP2; adenylate kinase-like protein 2; AP5A; P1,P5-di (adenosine-5â²) pentaphosphate; CRT; chloroquine resistance transporter; E. coli; Escherichia coli; GAK; GTP:AMP phosphot
A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation
Keywords: β-PIX; β-p21-activated kinase interacting exchange factor; Ca2 +; calcium; CaM; calmodulin; c-Cbl; casitas B-cell lymphoma; CC; coiled-coil; CD; circular dichroism; DH; diffuse B-cell lymphoma homology; EGFR; epidermal growth factor receptor; GEF; guan
The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter
Keywords: His-tag; polyhistidine tag; IC50; half maximum inhibitory concentration; IPTG; isopropyl-β-d-thiogalactoside; LB; Luria-Bertani growth media; DDM; n-dodecyl-β-d-maltoside; Ni-NTA; nickel-nitrilotriacetic acid; ORF; open reading frame; SDS-PAGE; sodium d
Relationship between stability and flexibility in the most flexible region of Photinus pyralis luciferase
Keywords: ATP; Adenosine triphosphate; ANS; 1-Anilino-8-naphthalene sulfonate; CD; Circular dichroism; Ni-NTA; Nickel nitrilotriacetic acid; Ppy; Photinus pyralis; Trp; Tryptophan; Bioluminescence; Luciferase; Thermostability; Flexibility; B-factor;
Identification and evaluation of anti Hepatitis C virus phytochemicals from Eclipta alba
Keywords: HCV; Hepatitis C virus; NS5B; non-structural protein 5B of hepatitis C virus; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; Poly (rA).(dT)18; polyriboadenylic acid annealed with (oligodeoxythymidylic acid)18; RdRp; RNA-dependant RNA
Molecular analysis of Plasmodium falciparum co-chaperone Aha1 supports its interaction with and regulation of Hsp90 in the malaria parasite
Keywords: 5-FOA; 5-fluoroorotic acid; ADP; adenosine diphosphate; Aha1; Activator of Hsp90 ATPase 1; ATP; adenosine triphosphate; Cub; C-terminal half of ubiquitin; DNA; deoxyribonucleic acid; GST; glutathione S-transferase; His6; hexa-histidine; Hsp90; Heat shock