کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8358704 | 1542075 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oligomerization of rice granule-bound starch synthase 1 modulates its activity regulation
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کلمات کلیدی
GSHELCdl-dithiothreitolPMSFAGPaseADPGDTNBCAPSSBEADP-glucose pyrophosphorylaseNi-NTAadenosine diphosphate glucoseGSTNADPHPPaseDTTIPTGNADPlambda protein phosphatasePVDFDBEOligomerizationDPANADPH-dependent thioredoxin reductase3-(cyclohexylamino)-1-propanesulfonic acid - 3- (cyclohexylamino) -1-propanesulfonic acid5,5′-dithiobis-(2-nitrobenzoic acid) - 5،5'-dithiobis- (2-nitrobenzoic acid)MAPK - MAPKγ-glutamylcysteine synthetase - γ-گلوتامیل سستئین سینتاتازstarch debranching enzyme - آنزیم انحلال نشاستهstarch branching enzyme - آنزیم شاخه ای نشاسته ای استRice - برنجEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاSpecific enzyme activity - فعالیت آنزیمی خاصPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدstarch synthase - نشاسته سنتازmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استPoly(vinylidene difluoride) - پلی وینیلیدین دی فلوئوریدreduced glutathione - کاهش گلوتاتیونEating quality - کیفیت غذا خوردنglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Granule-bound starch synthase 1 (GBSS1) is responsible for amylose synthesis in cereals, and this enzyme is regulated at the transcriptional and post-transcriptional levels. In this study, we show that GBSS1 from Oryza sativa L. (OsGBSS1) can form oligomers in rice endosperm, and oligomerized OsGBSS1 exhibits much higher specific enzymatic activity than the monomer. A monomer-oligomer transition equilibrium for OsGBSS1 occurs in the endosperm during development. Redox potential is a key factor affecting the oligomer percentage as well as the enzymatic activity of OsGBSS1. Adenosine diphosphate glucose, the direct donor of glucose, also impacts OsGBSS1 oligomerization in a concentration-dependent manner. OsGBSS1 oligomerization is influenced by phosphorylation status, which was strongly enhanced by Mitogen-activated protein kinase (MAPK) and ATP treatment and was sharply weakened by protein phosphatase (PPase) treatment. The activity of OsGBSS1 affects the ratio of amylose to amylopectin and therefore the eating quality of rice. Understanding the regulation of OsGBSS1 activity may lead to the improvement of rice eating quality.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 210, September 2013, Pages 141-150
Journal: Plant Science - Volume 210, September 2013, Pages 141-150
نویسندگان
De-Rui Liu, Wei-Xue Huang, Xiu-Ling Cai,