کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798204 | 1053305 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural changes in a binary mixed phospholipid bilayer of DOPG and DOPS upon saposin C interaction at acidic pH utilizing 31P and 2H solid-state NMR spectroscopy
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کلمات کلیدی
Sap CMLVsGCaseHClMALDI-TOFCP-MASSCDDOPGDOPSTFEdMPCPLLnuclear magnetic resonance - رزونانس مغناطیسی هستهایChemical shift anisotropy - Anisotropy شبیه سازی شیمیاییdioleoylphosphatidylglycerol - dioleoyl فسفاتیدیل گلیسرولmultilamellar vesicles - vesicles multilamellarhydrochloric acid - اسید هیدروکلریک یا اسید کلریدریک یا جوهر نمک CSA - ایالات مؤتلفهٔ آمریکاNMR - تشدید مغناطیسی هستهای Dioleoylphosphatidylserine - دیئولیل فسفاتیدیلزرینSaposin C - ساپوزین سیSolid-state NMR spectroscopy - طیف سنجی NMR جامداتphosphatidylglycerol - فسفاتیدیل گلیسرولPhosphatidylserine - فسفاتیدیلسرینmatrix assisted laser desorption ionization time-of-flight mass spectrometry - ماتریس با استفاده از طیف سنجی جرمی یونیزاسیون یونیزاسیون لیزر جذب می شودMolecular Order parameter - پارامتر سفارش مولکولیPoly(l-lysine) - پلی (لیزین)Cross-Polarization Magic-Angle Spinning - چرخش قطبی زاویه جادویی زاویه چرخشCardiolipin - کاردیولیپینhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراglucosylceramidase - گلوکوزیلسرامیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Saposin C (Sap C) is known to stimulate the catalytic activity of the lysosomal enzyme glucosylceramidase (GCase) that facilitates the hydrolysis of glucosylceramide to ceramide and glucose. Both Sap C and acidic phospholipids are required for full activity of GCase. In order to better understand this interaction, mixed bilayer samples prepared from dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylserine (DOPS) (5:3 ratio) and Sap C were investigated using 2H and 31P solid-state NMR spectroscopy at temperatures ranging from 25 to 50 °C at pH 4.7. The Sap C concentrations used to carry out these experiments were 0 mol%, 1 mol% and 3 mol% with respect to the phospholipids. The molecular order parameters (SCD) were calculated from the dePaked 2H solid-state NMR spectra of Distearoyl-d70-phosphatidylglycerol (DSPG-d70) incorporated with DOPG and DOPS binary mixed bilayers. The SCD profiles indicate that the addition of Sap C to the negatively charged phospholipids is concentration dependent. SCD profiles of 1 mol% of the Sap C protein show only a very slight decrease in the acyl chain order. However, the SCD profiles of the 3 mol% of Sap C protein indicate that the interaction is predominantly increasing the disorder in the first half of the acyl chain near the head group (C1-C8) indicating that the amino and the carboxyl termini of Sap C are not inserting deep into the DOPG and DOPS mixed bilayers. The 31P solid-state NMR spectra show that the chemical shift anisotropy (CSA) for both phospholipids decrease and the spectral broadening increases upon addition of Sap C to the mixed bilayers. The data indicate that Sap C interacts similarly with the head groups of both acidic phospholipids and that Sap C has no preference to DOPS over DOPG. Moreover, our solid-state NMR spectroscopic data agree with the structural model previously proposed in the literature [X. Qi, G.A. Grabowski, Differential membrane interactions of saposins A and C. Implication for the functional specificity, J. Biol. Chem. 276 (2001) 27010-27017] [1].
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1717, Issue 1, 10 November 2005, Pages 58-66
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1717, Issue 1, 10 November 2005, Pages 58-66
نویسندگان
Shadi Abu-Baker, Xiaoyang Qi, Justin Newstadt, Gary A. Lorigan,