کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799240 | 1054246 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells
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کلمات کلیدی
NMDCrm1Xrn1AGO17mGLMBDDX6CHXActDpri-miRNAsMicroRNA-143RIPADAPImicroRNA-145IgGDdx5hypoxia inducible factor 1 alpha subunitLPSIPZARGONAUTE 1DDX17PVDFDcp1aGAPDHIFN-β7-methylguanosine - 7-متیل گوانوزینHIF1a - HIF1hnRNPs - hnRNP هاMiR-145 - MIR-145Argonaute 2 - آرگونوت 2actinomycin D - اکتینومایسین DAgo2 - اکشن 2immunoglobulin G - ایمونوگلوبولین Ginterferon-beta - اینترفرون بتاbeta-actin - بتا آکتینprocessing bodies - بدن پردازشmiR-143 - به miR-143rapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدtumor necrosis factor-alpha - تومور نکروز عامل آلفاP-bodies - جسد PRISC - خطرdiamidino-2-phenylindole - دیامیدینو 2-فنیلینولHeterogeneous nuclear ribonucleoproteins - ریبونولوپروتئین های هسته ای ناهمگنradioimmunoprecipitation assay - سنجش radioimmunoprecipitationcycloheximide - سیکلوهایسیمیدActb - عملAU-rich elements - عناصر غنی AUTNF-α - فاکتور نکروز توموری آلفاnonsense-mediated mRNA decay - فرسودن mRNA ناشی از بی معنی استLeptomycin B - لپتومایسین Blipopolysaccharide - لیپوپلی ساکاریدRace - مسابقهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAARE - هستندPolyvinylidene fluoride - پلی وینیلیدین فلورایدpre-miRNAs - پیش از miRNAsnegative control - کنترل منفیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In various human malignancies, widespread dysregulation of microRNA (miRNA) expression is reported to occur and affects various cell growth programs. Recent studies suggest that the expression levels of miRNAs that act as tumor suppressors are frequently reduced in cancers because of chromosome deletions, epigenetical changes, aberrant transcription, and disturbances in miRNA processing. MiR-143 and -145 are well-recognized miRNAs that are highly expressed in several tissues, but down-regulated in most types of cancers. However, the mechanism of this down-regulation has not been investigated in detail. Here, we show that DEAD-box RNA helicase 6, DDX6 (p54/RCK), post-transcriptionally down-regulated miR-143/145 expression by prompting the degradation of its host gene product, NCR143/145 RNA. In human gastric cancer cell line MKN45, DDX6 protein was abundantly expressed and accumulated in processing bodies (P-bodies). DDX6 preferentially increased the instability of non-coding RNA, NCR143/145, which encompasses the miR-143/145 cluster, and down-regulated the expression of mature miR-143/145. In human monocytic cell line THP-1, lipopolysaccharide treatment promoted the assembly of P-bodies and down-regulated the expression of NCR143/145 and its miR-143/145 rapidly. In these cells, cycloheximide treatment led to a loss of P-bodies and to an increase in NCR143/145 RNA stability, thus resulting in up-regulation of miR-143/145 expression. These data demonstrate that DDX6 contributed to the control of NCR143/145 RNA stability in P-bodies and post-transcriptionally regulated miR-143/145 expression in cancer cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1829, Issue 10, October 2013, Pages 1102-1110
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1829, Issue 10, October 2013, Pages 1102-1110
نویسندگان
Akio Iio, Takeshi Takagi, Kohei Miki, Tomoki Naoe, Atsuo Nakayama, Yukihiro Akao,