کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799346 | 1054317 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of the promoter of the human glucose transporter 10 gene
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کلمات کلیدی
P1 artificial chromosomeEMSAUTRT2DMGLUTAP2TSPPACSp1 - SP1Electrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزDiabetes - بیماری قندrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGlucose transporter - حمل و نقل گلوکزSilencer - خاموش کنندهType 2 diabetes mellitus - دیابت نوع دوRace - مسابقهuntranslated region - منطقه غیر ترجمهtranscription start point - نقطه شروع رونویسیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The human SLC2A10 gene encodes the high-affinity glucose transporter 10 (GLUT10) and is widely expressed in adult tissues, including organs which play major roles in glucose homeostasis. Its function and genomic location in a region linked to Type 2 diabetes susceptibility are consistent with a potential role in Type 2 diabetes. Analysis of the CpG-rich promoter revealed the presence of two major transcription start points with differential use in tissues and cell lines. Mapping of transcriptionally active regions in the 5â² flanking sequence identified a region, located between nucleotides â70 and â14 (relative to the major transcription start point) as the SLC2A10 basal promoter. This sequence harbors consensus binding sites for Sp, AP2α, and other transcription factors. A juxtaposed Sp/AP2α motif located between â25 and â11 is critical for core promoter function. In cells expressing Sp and AP2 factors, the two motifs are required for maximal activation of the basal promoter. In cells lacking AP2α, transcription is dependent on the integrity of the Sp site. Using electrophoresis mobility shift assays, we demonstrate that Sp1 and Sp3 bind to the GC-box in site 5 forming specific complexes. In addition, a silencer region is present upstream of â696 which down-regulates SLC2A10 promoter activity independently of its distance to the transcript start site.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1730, Issue 2, 15 August 2005, Pages 147-158
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1730, Issue 2, 15 August 2005, Pages 147-158
نویسندگان
Fernando Segade, Dax C. Allred, Donald W. Bowden,