کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10801676 | 1055629 | 2016 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s)
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کلمات کلیدی
PEX5PEXAACIAAIBASAMERADROS - ROSalanine-glyoxylate aminotransferase - آلانین گلیوکسیلات آمینوترانسفرازindole-3-butyric acid - اسید Indole-3-Butyricindole-3-acetic acid - اسید Indole-3-استیکBinding domain - دامنه اتصالTPR - روش پاسخ فیزیکیendoplasmic reticulum - شبکه آندوپلاسمی Evolution - فرگشت sorting and assembly machinery - ماشین آلات مرتب سازی و مونتاژAmino acid content - محتوای آمینو اسیدAGT - هشتPeroxin - پراکسینperoxisome - پراکسیومProteome - پروتئومpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s) Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s)](/preview/png/10801676.png)
چکیده انگلیسی
Our knowledge of the proteome of plant peroxisomes and their functional plasticity is far from being complete, primarily due to major technical challenges in experimental proteome research of the fragile cell organelle. Several unexpected novel plant peroxisome functions, for instance in biotin and phylloquinone biosynthesis, have been uncovered recently. Nevertheless, very few regulatory and membrane proteins of plant peroxisomes have been identified and functionally described up to now. To define the matrix proteome of plant peroxisomes, computational methods have emerged as important powerful tools. Novel prediction approaches of high sensitivity and specificity have been developed for peroxisome targeting signals type 1 (PTS1) and have been validated by in vivo subcellular targeting analyses and thermodynamic binding studies with the cytosolic receptor, PEX5. Accordingly, the algorithms allow the correct prediction of many novel peroxisome-targeted proteins from plant genome sequences and the discovery of additional organelle functions. In this review, we provide an overview of methodologies, capabilities and accuracies of available prediction algorithms for PTS1 carrying proteins. We also summarize and discuss recent quantitative, structural and mechanistic information of the interaction of PEX5 with PTS1 carrying proteins in relation to in vivo import efficiency. With this knowledge, we develop a model of how proteins likely evolved peroxisomal targeting signals in the past and still nowadays, in which order the two import pathways might have evolved in the ancient eukaryotic cell, and how the secondary loss of the PTS2 pathway probably happened in specific organismal groups. This article is part of a Special Issue entitled: Peroxisomes edited by Ralf Erdmann.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 5, May 2016, Pages 790-803
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 5, May 2016, Pages 790-803
نویسندگان
S. Reumann, G. Chowdhary, T. Lingner,