کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10802941 | 1055745 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Divergent pathways for TNF and C2-ceramide toxicity in HTC hepatoma cells
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کلمات کلیدی
desipramineC2-CerTNFBAFA1dpmCHX3-MAC2-ceramideTNF-alphaDEX3-methyladenine - 3-متیل آدنینAcridine orange - آکاردین نارنجیFumo - استعمال دخانیاتacidic sphingomyelinase - اسفنجومیلیناز اسیدیbafilomycin A1 - بافیلومایسین A1Dexamethasone - دگزامتازونHepatoma cells - سلولهای هپاتوماcycloheximide - سیکلوهایسیمیدtumor necrosis factor - فاکتور نکروز تومورPhosphatidylserine - فسفاتیدیلسرینFumonisin B1 - فومونیزین B1Cell death - مرگ سلولی Propidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously showed that, in the rat hepatoma cell line HTC, TNF brings about a non-caspase-dependent, apoptosis-like process requiring NADPH oxidase activity, an iron-mediated pro-oxidant status, and a functional acidic vacuolar compartment. This process may thus involve mechanisms such as autophagy or relocation of lysosomal enzymes, perhaps secondary to the formation of ceramide by acidic sphingomyelinase. Here we investigated whether ceramide formation contributes to the apoptogenic process. HTC cells were found to be sensitive to exogenous ceramide and significantly protected against TNF by desipramine, an inhibitor of lysosomal acid sphingomyelinase. However, Bcl-2 transfection and Bcl-xL upregulation by dexamethasone significantly diminished the apoptogenic effect of ceramide but not that of TNF, suggesting that ceramide is not directly involved in TNF toxicity. Moreover, Bcl-xL silencing precluded dexamethasone-induced protection against ceramide and, by itself, induced massive death, demonstrating the strict dependence of HTC cells on Bcl-xL for survival also under standard culture conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1793, Issue 7, July 2009, Pages 1182-1190
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1793, Issue 7, July 2009, Pages 1182-1190
نویسندگان
Riccardo Autelli, Chiara Ullio, Elisa Prigione, Silvia Crepaldi, Nicola Schiavone, Ulf T. Brunk, Sergio Capaccioli, Francesco M. Baccino, Gabriella Bonelli,