کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803086 | 1055772 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Caveolae are an essential component of the pathway for endothelial cell signaling associated with abrupt reduction of shear stress
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کلمات کلیدی
DPIFITCDcfdichlorofluoresceinROS - ROSNADPH oxidase - اکسیداز NADPH Shear stress - تنش برشیCell proliferation - تکثیر سلولیdiphenyleneiodonium chloride - دی فینیلنیدونیم کلریدfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMechanotransduction - مکانیک انتقالKATP channel - کانال KATPReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Abrupt cessation of flow representing the acute loss of shear stress (simulated ischemia) to flow-adapted pulmonary microvascular endothelial cells (PMVEC) leads to reactive oxygen species (ROS) generation that signals for EC proliferation. We evaluated the role of caveolin-1 on this cellular response with mouse PMVEC that were preconditioned for 72Â h to laminar flow at 5Â dyn/cm2 followed by stop of flow (“ischemia”). Preconditioning resulted in a 2.7-fold increase in cellular expression of KATP (KIR 6.2) channels but no change in expression level of caveolin-1, gp91phox, or MAP kinases. The initial response to ischemia in wild type cells was cell membrane depolarization that was abolished by gene targeting of KIR 6.2. The subsequent response was increased ROS production associated with activation of NADPH oxidase (NOX2) and then phosphorylation of MAP kinases (Erk, JNK). After 24Â h of ischemia in wild type cells, the cell proliferation index increased 2.5 fold and the % of cells in SÂ +Â G2/M phases increased 6-fold. This signaling cascade (cell membrane depolarization, ROS production, MAP kinase activation and cell proliferation) was abrogated in caveolin-1 null PMVEC or by treatment of wild type cells with filipin. These studies indicate that caveolin-1 functions as a shear sensor in flow-adapted EC resulting in ROS-mediated cell signaling and endothelial cell proliferation following the abrupt reduction in flow.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 10, October 2008, Pages 1866-1875
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 10, October 2008, Pages 1866-1875
نویسندگان
Tatyana Milovanova, Shampa Chatterjee, Brian J. Hawkins, NanKang Hong, Elena M. Sorokina, Kris DeBolt, Jonni S. Moore, Muniswamy Madesh, Aron B. Fisher,