Turning pyridoxal-5′-phosphate-dependent enzymes into thermostable binding proteins: d-Serine dehydratase from baker's yeast as a case study

► Reduction of the aldimine linkage greatly increases Dsd thermal stability. ► No changes occur to the secondary structure following reduction. ► Reduced Dsd retains the ability to bind d-serine enantioselectively. ► Reduced Dsd does not consume its substrate.