کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815008 | 1058441 | 2016 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comprehensive analysis of chemokine-induced cAMP-inhibitory responses using a real-time luminescent biosensor
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کلمات کلیدی
EpacPDEFskPGE2forskolinpKaAPCAUC - AUCcAMP - cAMPadenylate cyclase - آدنیلات سیکلاسallophycocyanin - آلوفوکسیانینBiosensor - بیوسنسورphycoerythrin - فایکوئیریدینPhosphodiesterase - فسفو دی استرازarea under curve - منطقه تحت منحنیExchange protein directly activated by cAMP - پروتئین تبادل شده به طور مستقیم توسط cAMP فعال می شودprotein kinase A - پروتئین کیناز AProstaglandin E2 - پروستاگلاندین E2Chk - چاکChemokine - کموکاین یا کموکین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chemokine receptors are members of the G-protein-coupled receptor (GPCR) family coupled to members of the Gi class, whose primary function is to inhibit the cellular adenylate cyclase. We used a cAMP-related and PKA-based luminescent biosensor (GloSensor⢠F-22) to monitor the real-time downstream response of chemokine receptors, especially CX3CR1 and CXCR4, after activation with their cognate ligands CX3CL1 and CXCL12. We found that the amplitudes and kinetic profiles of the chemokine responses were conserved in various cell types and were independent of the nature and concentration of the molecules used for cAMP prestimulation, including either the adenylate cyclase activator forskolin or ligands mediating Gs-mediated responses like prostaglandin E2 or beta-adrenergic agonist. We conclude that the cAMP chemokine response is robustly conserved in various inflammatory conditions. Moreover, the cAMP-related luminescent biosensor appears as a valuable tool to analyze the details of Gi-mediated cAMP-inhibitory cellular responses, even in native conditions and could help to decipher their precise role in cell function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 120-129
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 120-129
نویسندگان
Virginia Felouzis, Patricia Hermand, Guy Trambly de Laissardière, Christophe Combadière, Philippe Deterre,