Myocyte enhancer factor 2 (MEF2) tethering to muscle selective A-kinase anchoring protein (mAKAP) is necessary for myogenic differentiation

► MEF2 and mAKAP interact directly in C2C12 cells during myogenic differentiation. ► This interaction is required for transcriptional activity of MEF2. ► Disruption of the interaction halts myogenic differentiation. ► Disruption of the interaction blunts expression of MEF2 target genes.