کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815738 | 1058500 | 2010 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lysophosphatidic acid induces upregulation of Mcl-1 and protects apoptosis in a PTX-dependent manner in H19-7 cells
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کلمات کلیدی
PI3KNeuronal progenitor cellmyeloid cell leukemiaLPAGSK-3NPCS1PPTX5-bromodeoxyuridineGPCRqRT-PCRMCLMcl-1CREBDMEMFBSERKFGFBSA - BSAG-protein coupled receptor - G-پروتئین همراه گیرندهMAPK - MAPKDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوsphingosine 1-phosphate - اسپینگزین 1-فسفاتlysophosphatidic acid - اسید لیسفسفیدیدBrdU - بروموداکسی اوریدینCell survival - بقای سلولیembryonic day - روز جنینیfetal bovine serum - سرم جنین گاوpertussis toxin - سموم سورافنیfibroblast growth factor - فاکتور رشد فیبروبلاستphosphatidylinositol-3 kinase - فسفاتیدیلینوزیتول 3 کینازNeurogenesis - نوروژنزcAMP response element binding protein - پروتئین اتصال دهنده عنصر پاسخ cAMPmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیglycogen synthase kinase 3 - گلیکوزین سنتاز کیناز 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Lysophosphatidic acid (LPA) is a lipid growth factor known to regulate diverse cell functions, including cell proliferation, survival, and apoptosis. Tight regulation of cell survival in neuronal precursor is essential during neurogenesis in both developing and adult brain. Increasing data show that diverse external factors including LPA play roles in controlling cell survival and apoptosis in early developing neurons. However, the underlying control mechanism remains unclear. To explore how LPA regulates cell survival or apoptosis in a developing neuron, mechanisms for cell survival and signaling cascades by LPA were investigated in H19-7 hippocampal progenitor cells. Here, we showed that LPA promotes cell survival by protection from apoptosis. Mcl-1 was demonstrated to be crucial in LPA-induced cell survival by transfection of the siRNA specific for Mcl-1 and overexpression of Mcl-1. LPA-induced cell survival was critically mediated by the upregulation of Mcl-1 which was regulated not only through a post-translational control but a transcriptional control. Mcl-1 stabilization by LPA-induced inhibitory phosphorylation of GSK-3 contributed predominantly to the Mcl-1 upregulation. Both LPA-induced cell survival and the GSK-3 phosphorylation were attenuated by PTX and by siRNA specific for LPA1 or LPA2 receptor. Taken together, these results showed that Mcl-1 stabilization by inhibitory phosphorylation of GSK-3 through Gi/o coupling of the LPA1 and LPA2 receptors following Mcl-1 upregulation plays a critical role in LPA-induced survival of H19-7 cells. In developing neurons, modulation of Mcl-1 levels may constitute a crucial mechanism for controlling their fates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 22, Issue 3, March 2010, Pages 484-494
Journal: Cellular Signalling - Volume 22, Issue 3, March 2010, Pages 484-494
نویسندگان
Yuanjie Sun, Ju-Suk Nam, Dong-Hoon Han, Nam-Ho Kim, Ho-Kyew Choi, Jeong Kug Lee, Hae Jin Rhee, Sung-Oh Huh,