کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815966 | 1058530 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cannabinoid receptor interacting protein (CRIP1a) attenuates CB1R signaling in neuronal cells
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کلمات کلیدی
PBSG protein coupled receptor kinaseSp-cAMPSThlCP55940SR141716CRIP1aMAEAWIN55212-2GRK2-arachidonoylglycerolGPCRCB12-AGDTTBSA - BSAcAMP - cAMPERK1/2 - ERK1 / 2G protein coupled receptor - G پروتئین همراه با پروتئینMAPK - MAPKDAGL - NEC روزانهTetrahydrolipstatin - tetrahydrolipstatinbovine serum albumin - آلبومین سرم گاوendocannabinoid - آندوکانابینوئیدG protein - جی پروتئینdynasore - دیوانهdithiothreitol - دیتیوتریتولPhosphate buffered saline - فسفات بافر شورdiacylglycerol lipase - لیپاز دی سیل گلیسرولmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinases 1 and 2 - کینازهای 1 و 2 تنظیم شده سیگنال خارج سلولیCB1 receptor - گیرنده CB1cannabinoid receptor - گیرنده های کانابینوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CB1 cannabinoid receptors (CB1R) are one of the most abundantly expressed G protein coupled receptors (GPCR) in the CNS and regulate diverse neuronal functions. The identification of GPCR interacting proteins has provided additional insight into the fine-tuning and regulation of numerous GPCRs. The cannabinoid receptor interacting protein 1a (CRIP1a) binds to the distal carboxy terminus of CB1R, and has been shown to alter CB1R-mediated neuronal function [1]. The mechanisms by which CRIP1a regulates CB1R activity have not yet been identified; therefore the focus of this investigation is to examine the cellular effects of CRIP1a on CB1R signaling using neuronal N18TG2 cells stably transfected with CRIP1a over-expressing and CRIP1a knockdown constructs. Modulation of endogenous CRIP1a expression did not alter total levels of CB1R, ERK, or forskolin-activated adenylyl cyclase activity. When compared to WT cells, CRIP1a over-expression reduced basal phosphoERK levels, whereas depletion of CRIP1a augmented basal phosphoERK levels. Stimulation of phosphoERK by the CB1R agonists WIN55212-2, CP55940 or methanandamide was unaltered in CRIP1a over-expressing clones compared with WT. However, CRIP1a knockdown clones exhibited enhanced ERK phosphorylation efficacy in response to CP55940. In addition, CRIP1a knockdown clones displayed a leftward shift in CP55940-mediated inhibition of forskolin-stimulated cAMP accumulation. CB1R-mediated Gi3 and Go activation by CP99540 was attenuated by CRIP1a over-expression, but robustly enhanced in cells depleted of CRIP1a. Conversely, CP55940-mediated Gi1 and Gi2 activation was significant enhanced in cells over-expressing CRIP1a, but not in cells deficient of CRIP1a. These studies suggest a mechanism by which endogenous levels of CRIP1a modulate CB1R-mediated signal transduction by facilitating a Gi/o protein subtype preference for Gi1 and Gi2, accompanied by an overall suppression of G-protein-mediated signaling in neuronal cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 27, Issue 3, March 2015, Pages 716-726
Journal: Cellular Signalling - Volume 27, Issue 3, March 2015, Pages 716-726
نویسندگان
Lawrence C. Blume, Khalil Eldeeb, Caroline E. Bass, Dana E. Selley, Allyn C. Howlett,