کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10816511 | 1058576 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Disruption of microtubules leads to glucocorticoid receptor degradation in HeLa cell line
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کلمات کلیدی
DEXJnkmDCsDTTGCsGRECytochromes P450AP-1BSA - BSAc-Jun N-terminal kinase - C-Jun N-terminal kinaseCYPs - CYPbovine serum albumin - آلبومین سرم گاوDexamethasone - دگزامتازونdithiothreitol - دیتیوتریتولglucocorticoid responsive element - عنصر واکنش گلوکوکورتیکوئیدCAR - ماشینactivator protein 1 - پروتئین فعال کننده 1Colchicine - کلشی سینcol - کولGlucocorticoids - گلوکوکورتیکوئیدهاconstitutive androstane receptor - گیرنده آندروستان پایدار
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The role of microtubules (MTs) in steroid hormone-dependent human glucocorticoid receptor (hGR) activation/translocation is controversial. It was demonstrated recently that colchicine (COL) down-regulates hGR-driven genes in primary human hepatocytes by a mechanism involving inhibition of hGR translocation to the nucleus. To investigate whether inhibition of hGR translocation is the sole reason for its inactivation, we used human cervical carcinoma cells (HeLa) as a model. Herein we present evidence that perturbation of microtubules in HeLa cells leads to rapid time- and dose-dependent degradation of hGR protein. Degradation is proteasome mediated as revealed by its reversibility by proteasome inhibitor MG132. Moreover, degradation was observed for neither wt-hGR nor hGR mutants S226A and K419A in transiently transfected COS-1 cells. On the other hand, c-jun N-terminal kinase (JNK) seems not to be involved in the process because JNK inhibitor 1,9-Pyrazoloanthrone (SP600125) does not reverse hGR degradation. Similarly, another hGR functional antagonist, nuclear factor kappa beta (NFκB), did not play any role in the degradation process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 17, Issue 2, February 2005, Pages 187-196
Journal: Cellular Signalling - Volume 17, Issue 2, February 2005, Pages 187-196
نویسندگان
ZdenÄk DvoÅák, Martin Modrianský, Jitka Ulrichová, Patrick Maurel, Marie-Jose Vilarem, Jean-Marc Pascussi,