کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840132 | 1067570 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Response of the ascorbate-glutathione cycle to re-aeration following hypoxia in lupine roots
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کلمات کلیدی
monodehydroascorbatemonodehydroascorbate reductasedehydroascorbateGSSGMDHARASAdehydroascorbate reductaseDHARCATAPXGSHROS - ROSHydrogen peroxide - آب اکسیژنهreduced ascorbate - آسکوربات کاهش یافته استMdha - بزرگترDHA - دوکوساهگزائنوئیک اسیدdihydroethidium - دی هیدروتیدیمSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازH2O2 - هیدروژن پراکسیدHypoxia - هیپوکسیDHE - وascorbate peroxidase - پراکسیداز آسکورباتیAscorbate–glutathione cycle - چرخه اسکوربات-گلوتاتیونCatalase - کاتالازreduced glutathione - کاهش گلوتاتیونoxidized glutathione - گلوتاتیون اکسید شدهglutathione reductase - گلوتاتیون ردوکتازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Response of the ascorbate-glutathione cycle to re-aeration following hypoxia in lupine roots Response of the ascorbate-glutathione cycle to re-aeration following hypoxia in lupine roots](/preview/png/10840132.png)
چکیده انگلیسی
The response of the enzymes and metabolites of the ascorbate-glutathione pathway to oxidative stress caused by re-aeration following hypoxia was studied in roots of hydroponically grown lupine (Lupinus luteus L. cv. Juno) seedlings. Lupine roots were deprived of oxygen by subjecting them to hypoxia for 48 and 72Â h and then re-aerated for up to 4Â h. An increased content of total ascorbate was observed in lupine roots immediately after hypoxia, whereas total glutathione level decreased. However, a significant increase in the reduced forms of both metabolites was found directly after hypoxia. Re-admission of oxygen caused the decrease of the ratios of reduced to oxidized forms of ascorbate and glutathione, indicating oxidative stress. While monodehydroascorbate reductase (MDHAR, EC 1.6.5.4) activity remained unaltered during re-aeration the increase in activities of ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) was observed 30Â min after transfer from hypoxic condition. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) activity approached the control level during a whole re-aeration period. Native gel electrophoresis combined with specific activity staining revealed seven isoforms of APX, five isoforms of GR and three different proteins with DHA reductase activity in roots extracts. However, immediately after hypoxic treatment APX-5 isoform and GR-1 isoform were not observed in roots. This experimental system was also used to investigate superoxide anion level in roots utilizing the superoxide anion-specific indicator dihydroethidium (DHE). Intense DHE-derived fluorescence was found in re-aerated root tips as compared to control roots, indicating that re-aeration induced superoxide anion production in hypoxically pretreated roots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 6, June 2005, Pages 583-590
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 6, June 2005, Pages 583-590
نویسندگان
MaÅgorzata Garnczarska,