Expression, purification, and reconstitution of the transmembrane domain of the human amyloid precursor protein for NMR studies

► APPTM has been successfully overexpressed as a fusion protein with MBP. ► Pure APPTM can be obtained by Ni-NTA chromatography, TEV protease cleavage and RP-HPLC. ► Isotopically labeled APPTM WT and an FAD mutant were purified for NMR studies. ► Excellent HSQC spectra were obtained in DPC and SDS micelles.