Prostate cancer cells metabolize d-lactate inside mitochondria via a d-lactate dehydrogenase which is more active and highly expressed than in normal cells

► First evidence is given that cancer cells metabolize d-lactate inside mitochondria. ► d-Lactate dehydrogenase (d-LDH) is shown to be present in prostate mitochondria. ► d-LDH protein level and activity is higher in cancer than in normal prostate cells. ► d-Lactate metabolism occurs with membrane potential generation and ATP synthesis. ► d-Lactate enters mitochondria and causes malate export at a higher rate in cancer.