کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10889827 1081500 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
UPLC/MS based method for quantitative determination of fatty acid composition in Gram-negative and Gram-positive bacteria
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
UPLC/MS based method for quantitative determination of fatty acid composition in Gram-negative and Gram-positive bacteria
چکیده انگلیسی
Quantitative fatty acid composition of microorganisms at various growth space points is required for understanding membrane associated processes of cells, but the majority of the relevant publications still restrict to the relative compositions. In the current study, a simple and reliable method for quantitative measurement of fatty acid content in bacterial biomass without prior derivatization using ultra performance liquid chromatography-electrospray ionization mass spectrometry was developed. The method was applied for investigating the influence of specific growth rate and pH on the fatty acid profiles of two biotechnologically important microorganisms - Gram-negative bacteria Escherichia coli and Gram-positive bacteria Lactococcus lactis grown in controlled physiological states. It was found that the membranes of slowly growing cells are more rigid and that the fatty acid fraction of the cells of L. lactis diminishes considerably with increasing growth rate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 82, Issue 3, September 2010, Pages 288-295
نویسندگان
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