کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903815 | 1086529 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LIM homeobox transcription factor Lhx2 inhibits skeletal muscle differentiation in part via transcriptional activation of Msx1 and Msx2
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کلمات کلیدی
PBSEMSAMsx2Lhx2MyHCMSX1FGF-2BPSBMP4RT-PCRTGFβGAPDHDMEMFBSDulbecco׳s modified Eagle׳s medium - Dilbecco's modified Eagle's mediumshort interfering RNA - RNA تداخل کوتاهsiRNA - siRNAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینTransforming Growth Factor Beta - تبدیل بتا فاکتور رشدSkeletal muscle differentiation - تمایز عضلانی اسکلتیbase pairs - جفت پایهLimb bud - جوانه اندامreverse transcription PCR - رونویسی معکوس PCRMyosin heavy chain - زنجیره سنگین میوزینfetal bovine serum - سرم جنین گاوES cells - سلول ESEmbryonic stem cells - سلولهای بنیادی جنینیfibroblast growth factor-2 - فیبروبلاست رشد فاکتور 2Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریbone morphogenetic protein 4 - پروتئین مورفوژنیک استخوان 4Penicillin-streptomycin - پنیسیلین- استرپتومایسینCHiP - چیپglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
LIM homeobox transcription factor Lhx2 is known to be an important regulator of neuronal development, homeostasis of hair follicle stem cells, and self-renewal of hematopoietic stem cells; however, its function in skeletal muscle development is poorly understood. In this study, we found that overexpression of Lhx2 completely inhibits the myotube-forming capacity of C2C12 cells and primary myoblasts. The muscle dedifferentiation factors Msx1 and Msx2 were strongly induced by the Lhx2 overexpression. Short interfering RNA-mediated knockdown of Lhx2 in the developing limb buds of mouse embryos resulted in a reduction in Msx1 and Msx2 mRNA levels, suggesting that they are downstream target genes of Lhx2. We found two Lhx2 consensus-binding sites in the â2097 to â1189 genomic region of Msx1 and two additional sites in the â536 to +73 genomic region of Msx2. These sequences were shown by luciferase reporter assay to be essential for Lhx2-mediated transcriptional activation. Moreover, electrophoretic mobility shift assays and chromatin immunoprecipitation assays showed that Lhx2 is present in chromatin DNA complexes bound to the enhancer regions of the Msx1 and Msx2 genes. These data demonstrate that Msx1 and Msx2 are direct transcriptional targets of Lhx2. In addition, overexpression of Lhx2 significantly enhanced the mRNA levels of bone morphogenetic protein 4 and transforming growth factor beta family genes. We propose that Lhx2 is involved in the early stage of skeletal muscle development by inducing multiple differentiation inhibitory factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 331, Issue 2, 15 February 2015, Pages 309-319
Journal: Experimental Cell Research - Volume 331, Issue 2, 15 February 2015, Pages 309-319
نویسندگان
Yusaku Kodaka, Kiyoko Tanaka, Kenji Kitajima, Kosuke Tanegashima, Ryoichi Matsuda, Takahiko Hara,