کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903902 | 1086539 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
SUV420H2 suppresses breast cancer cell invasion through down regulation of the SH2 domain-containing focal adhesion protein tensin-3
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کلمات کلیدی
H4K20me3mAbserine/threonine-protein kinaseFSTPlk2PABPTBSH2IL7RH4K20me1Monoclonal antibody - آنتی بادی مونوکلونالPolyclonal antibody - آنتی بادی های پلی کلونالchromatin immunoprecipitation - ایمن سازی کروماتینInterleukin-24 - اینترلوکین -24Breast tumor - تومور پستانCell invasion - حمله سلولیphosphotyrosine-binding - فسفاتیزین اتصالSrc Homology 2 - همبستگی Src 2CHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The genome-wide loss of histone H4 lysine 20 tri-methylation (H4K20me3) is observed in multiple types of cancer, including breast tumors. Since H4K20me3 is preferentially targeted to repetitive elements in the pericentromeric and telomeric heterochromatin and plays a role in chromatin integrity, the pathological effects of disrupted H4K20me3 in tumors have been attributed to genomic instability. However, in this report, we show that loss of H4K20me3 modulates gene expression profiles, leading to increased cell invasion. Reduced H4K20me3 levels in tumor cells are often accompanied by a decrease in the expression of the H4K20-specific methyltransferase, SUV420H2. Exogenous delivery of SUV420H2 into MDA-MB-231 human breast cancer cells induced selective and specific changes in the expression of cancer-related genes. One of the most downregulated genes in response to SUV420H2 expression was the Src substrate, tensin-3, a focal adhesion protein that contributes to cancer cell migration. Depletion of tensin-3 suppressed breast cancer cell invasiveness. Furthermore, silencing of tensin-3 was associated with enrichment of H4K20me3 immediately upstream of the tensin-3 transcription start site, suggesting that the loss of H4K20me3 in tumor cells induced the expression of cancer-promoting genes. These findings connect the loss of H4K20me3 with tumor progression, through the transcriptional activation of cancer-promoting genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 334, Issue 1, 15 May 2015, Pages 90-99
Journal: Experimental Cell Research - Volume 334, Issue 1, 15 May 2015, Pages 90-99
نویسندگان
Yoshimi Shinchi, Miki Hieda, Yu Nishioka, Ayaka Matsumoto, Yuhki Yokoyama, Hiroshi Kimura, Shuji Matsuura, Nariaki Matsuura,