کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904078 | 1086556 | 2014 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation-induced cytidine deaminase (AID) is localized to subnuclear domains enriched in splicing factors
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کلمات کلیدی
CSRFIBU2AF65SMNsnRNPNLSSHMSC35Activation-induced cytidine deaminasesomatic hypermutation - ابرمتن سوتیclass switch recombination - بازوی سوئیچ کلاسCajal bodies - بدن کجالCajal body - بدن کجالsmall nuclear ribonucleoprotein - ریبونولوپروتئین کوچک هسته ایSurvival of motor neuron - زنده ماندن از نورون موتورNuclear speckles - ستاره های هسته ایnuclear localization signal - سیگنال محلی سازی هسته ایFibrillarin - فیبردارلینLeptomycin B - لپتومایسین BWestern blot - وسترن بلاتSm protein - پروتئین اسمAID - کمک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Activation-induced cytidine deaminase (AID) is the mutator enzyme in adaptive immunity. AID initiates the antibody diversification processes in activated B cells by deaminating cytosine to uracil in immunoglobulin genes. To some extent other genes are also targeted, which may lead to genome instability and B cell malignancy. Thus, it is crucial to understand its targeting and regulation mechanisms. AID is regulated at several levels including subcellular compartmentalization. However, the complex nuclear distribution and trafficking of AID has not been studied in detail previously. In this work, we examined the subnuclear localization of AID and its interaction partner CTNNBL1 and found that they associate with spliceosome-associated structures including Cajal bodies and nuclear speckles. Moreover, protein kinase A (PKA), which activates AID by phosphorylation at Ser38, is present together with AID in nuclear speckles. Importantly, we demonstrate that AID physically associates with the major spliceosome subunits (small nuclear ribonucleoproteins, snRNPs), as well as other essential splicing components, in addition to the transcription machinery. Based on our findings and the literature, we suggest a transcription-coupled splicing-associated model for AID targeting and activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 322, Issue 1, 10 March 2014, Pages 178-192
Journal: Experimental Cell Research - Volume 322, Issue 1, 10 March 2014, Pages 178-192
نویسندگان
Yi Hu, Ida Ericsson, Berit Doseth, Nina B. Liabakk, Hans E. Krokan, Bodil Kavli,