کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904277 | 1086570 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antibody-based analysis reveals “filamentous vs. non-filamentous” and “cytoplasmic vs. nuclear” crosstalk of cytoskeletal proteins
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کلمات کلیدی
nESeGFPNPCNLSLMBMonoclonal antibody - آنتی بادی مونوکلونالNuclear transport - حمل و نقل هسته ایNuclear scaffold - داربست هسته ایnuclear export signal - سیگنال صادرات هسته ایnuclear localization signal - سیگنال محلی سازی هسته ایLeptomycin B - لپتومایسین BNuclear matrix - ماتریس هسته ایnuclear pore complex - مجتمع مخرب هسته ایCytoskeleton - چارچوب یاخته، سیتواسکلتون، اسکلت سلولیKeratin - کراتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
To uncover the molecular composition and dynamics of the functional scaffold for the nucleus, three fractions of biochemically-stable nuclear protein complexes were extracted and used as immunogens to produce a variety of monoclonal antibodies. Many helix-based cytoskeletal proteins were identified as antigens, suggesting their dynamic contribution to nuclear architecture and function. Interestingly, sets of antibodies distinguished distinct subcellular localization of a single isoform of certain cytoskeletal proteins; distinct molecular forms of keratin and actinin were found in the nucleus. Their nuclear shuttling properties were verified by the apparent nuclear accumulations under inhibition of CRM1-dependent nuclear export. Nuclear keratins do not take an obvious filamentous structure, as was revealed by non-filamentous cytoplasmic keratin-specific monoclonal antibody. These results suggest the distinct roles of the helix-based cytoskeletal proteins in the nucleus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 319, Issue 20, 10 December 2013, Pages 3226-3237
Journal: Experimental Cell Research - Volume 319, Issue 20, 10 December 2013, Pages 3226-3237
نویسندگان
Masahiro Kumeta, Yuya Hirai, Shige H. Yoshimura, Tsuneyoshi Horigome, Kunio Takeyasu,