Modeling neurogenesis impairment in down syndrome with induced pluripotent stem cells from Trisomy 21 amniotic fluid cells

► We generated human Down syndrome iPS cells from Trisomy 21 amniotic fluid cells. ► We modeled neurogenesis impairment by neuronal progenitor cell from T21 iPS cells. ► In T21-NPCs, APP levels were increased, and MeCP2 levels are decreased. ► In T21-NPCs, the levels of 5 miRNAs located on chromosome 21 were increased. ► T21-NPCs exhibited a developmentally regulated defect in neurogenesis.