کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904966 | 1086713 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
p85 β-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase
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کلمات کلیدی
PIDFRNKFAK-related non-kinasep21 activated kinasePIXLysoPLDAutotaxinGIT1LPAATXPAKS1PFAKbFGFPI3Kp38 MAP kinase - p38 mAP kinaseSphingosine-1-phosphate - اسپینگسین-1-فسفاتlysophosphatidic acid - اسید لیسفسفیدیدbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهphosphatidylinositide 3-kinase - فسفاتیدیلینوزیتید 3-کینازlysophospholipase D - لیسفسفلیپاز Dfocal adhesion kinase - کیناز چسبندگی کانونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: p85 β-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase p85 β-PIX is required for cell motility through phosphorylations of focal adhesion kinase and p38 MAP kinase](/preview/png/10904966.png)
چکیده انگلیسی
Lysophosphatidic acid (LPA) mediates diverse biological responses, including cell migration, through the activation of G-protein-coupled receptors. Recently, we have shown that LPA stimulates p21-activated kinase (PAK) that is critical for focal adhesion kinase (FAK) phosphorylation and cell motility. Here, we provide the direct evidence that p85 β-PIX is required for cell motility of NIH-3T3 cells by LPA through FAK and p38 MAP kinase phosphorylations. LPA induced p85 β-PIX binding to FAK in NIH-3T3 cells that was inhibited by pretreatment of the cells with phosphoinositide 3-kinase inhibitor, LY294002. Furthermore, the similar inhibition of the complex formation was also observed, when the cells were transfected with either p85 β-PIX mutant that cannot bind GIT or dominant negative mutants of Rac1 (N17Rac1) and PAK (PAK-PID). Transfection of the cells with specific p85 β-PIX siRNA led to drastic inhibition of LPA-induced FAK phosphorylation, peripheral redistribution of p85 β-PIX with FAK and GIT1, and cell motility. p85 β-PIX was also required for p38 MAP kinase phosphorylation induced by LPA. Finally, dominant negative mutant of Rho (N19Rho)-transfected cells did not affect PAK activation, while the cells stably transfected with p85 β-PIX siRNA or N17Rac1 showed the reduction of LPA-induced PAK activation. Taken together, the present data suggest that p85 β-PIX, located downstream of Rac1, is a key regulator for the activations of FAK or p38 MAP kinase and plays a pivotal role in focal complex formation and cell motility induced by LPA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 307, Issue 2, 15 July 2005, Pages 315-328
Journal: Experimental Cell Research - Volume 307, Issue 2, 15 July 2005, Pages 315-328
نویسندگان
Jangsoon Lee, In Duk Jung, Won Keun Chang, Chang Gyo Park, Do Yeun Cho, Eun-Young Shin, Dong Wan Seo, Yong Kee Kim, Hyang Woo Lee, Jeung-Whan Han, Hoi Young Lee,