کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905045 | 1086718 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ligand depletion negatively controls the mitogenic activity of epidermal growth factor
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کلمات کلیدی
EGFPBSDMEMNCSTGFαMitogenic stimulationBSA - BSADulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوtransforming growth factor-alpha - تبدیل فاکتور رشد آلفاReceptor internalisation - درونی سازی گیرندهnewborn calf serum - سرم گاو تازه متولد شدهepidermal growth factor - عامل رشد اپیدرمیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmap - نقشهmitogen-activated protein - پروتئین فعال mitogen
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
EGF activates the ErbB1 receptor, but there appears only a limited correlation between its receptor binding affinity and mitogenic activity. This is indicated by our present observation that in cells with high ErbB1 expression, including SUM102 breast tumor cells, low affinity EGF/Notch chimeras have similarly high mitogenic activity as EGF, in spite of the fact that EGF is superior in inducing receptor tyrosine phosphorylation and p42/p44 MAP-kinase activity. However, as a result of receptor-mediated internalisation high-affinity ligands such as EGF are depleted much more rapidly from the extracellular medium than low-affinity EGF/Notch chimeras. As a consequence, the mitogenic activity of EGF on ErbB1 overexpressing cells is limited by substantial degradation of internalised ligand in the period before cells enter S-phase, a phenomenon that is not observed for low affinity mutant ligands. The mitogenic activity of EGF on ErbB1 overexpressing cells does therefore not only depend on the applied concentration but also on the total amount of ligand added, and is strongly underestimated when tested in a limited assay volume. No such dependence on the incubation volume was observed for EGF activity on cells with low ErbB1 expression levels and on cells for which EGF is growth inhibitory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 304, Issue 2, 1 April 2005, Pages 630-641
Journal: Experimental Cell Research - Volume 304, Issue 2, 1 April 2005, Pages 630-641
نویسندگان
Monique L.M. van de Poll, Walter van Rotterdam, Mireille M. Gadellaa, Saskia Jacobs-Oomen, Everardus J.J. van Zoelen,