کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10905185 1086737 2005 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beclin 1 augmented cis-diamminedichloroplatinum induced apoptosis via enhancing caspase-9 activity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Beclin 1 augmented cis-diamminedichloroplatinum induced apoptosis via enhancing caspase-9 activity
چکیده انگلیسی
Beclin 1, identified as a Bcl-2-interacting protein, is known to enhance autophagy. However, the effect of Beclin 1 on apoptotic signaling has remained unclear. Here, we show that overexpression of Beclin 1 in MKN28 human gastric cancer cells augmented cis-diamminedichloroplatinum (CDDP)-induced apoptosis. Conversely, “knockdown” of Beclin 1 by a small inhibitory RNA in MKN 1 cells attenuated this cytotoxicity. Furthermore, not only caspase-3/7 activities, but also caspase-9 activity was increased in Beclin 1 gene transfectants treated with CDDP, and caspase-9 inhibitor completely abolished augmentation of CDDP-induced apoptosis by Beclin 1 as did a caspase-3 inhibitor. Thus, Beclin 1 augments CDDP-induced apoptosis through enhancing caspase-9 activity and functions as a pro-apoptotic molecule.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 307, Issue 1, 1 July 2005, Pages 26-40
نویسندگان
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