کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905601 | 1086761 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
δ-Sarcoglycan is required for early zebrafish muscle organization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Mutations in sarcoglycans (α-, β-, γ-, and δ-) have been linked with limb girdle muscular dystrophy (LGMD) types 2C-F in humans. We have cloned the zebrafish orthologue encoding δ-sarcoglycan and mapped the gene to linkage group 21. The predicted zebrafish δ-sarcoglycan protein is highly homologous with its human orthologue including conservation of two of the three predicted glycosylation sites. Like other members of the dystrophin-associated protein complex (DAPC), δ-sarcoglycan localizes to the sarcolemmal membrane of the myofiber in adult zebrafish, but is more apparent at the myosepta in developing embryos. Zebrafish embryos injected with morpholinos against δ-sarcoglycan were relatively inactive at 5 dpf, their myofibers were disorganized, and swim bladders uninflated. Immunohistochemical and immunoblotting experiments show that δ-, β-, and γ-sarcoglycans were all downregulated in the morphants, whereas dystrophin expression was unaffected. Whereas humans lacking δ-sarcoglycan primarily show adult phenotypes, our results suggest that δ-sarcoglycan plays a role in early zebrafish muscle development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 304, Issue 1, 10 March 2005, Pages 105-115
Journal: Experimental Cell Research - Volume 304, Issue 1, 10 March 2005, Pages 105-115
نویسندگان
Jeffrey R. Guyon, Alycia N. Mosley, Susan J. Jun, Federica Montanaro, Leta S. Steffen, Yi Zhou, Vincenzo Nigro, Len I. Zon, Louis M. Kunkel,