کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10933478 | 1093799 | 2009 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased IP3/Ca2+ signaling compensates depletion of LET-413/DLG-1 in C. elegans epithelial junction assembly
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کلمات کلیدی
CeAJGOFEMOLOFMTSIP3NEBDDACdsRNAGUKInositol 1,4,5-triphosphate - Inositol 1،4،5-تری فسفاتdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهMAGUK - آنهاjunction - اتصالLoss-of-function - از دست دادن عملکردSpermatheca - اسپرماتیکاepithelia - اپیتلیاNuclear envelope breakdown - تجزیه پاکت هسته ایovulation - تخمک گذاریgain-of-function - سود عملکردCell polarity - قطب سلولیMicrotubules - میکروتوبول، ریزلوله هاCaenorhabditis elegans - کرم الگانس Gonadogenesis - گنادوژنزGuanylate kinase - گینیلات کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The let-413/scribble and dlg-1/discs large genes are key regulators of epithelial cell polarity in C. elegans and other systems but the mechanism how they organize a circumferential junctional belt around the apex of epithelial cells is not well understood. We report here that IP3/Ca2+ signaling is involved in the let-413/dlg-1 pathway for the establishment of epithelial cell polarity during the development in C. elegans. Using RNAi to interfere with let-413 and dlg-1 gene functions during post-embryogenesis, we discovered a requirement for LET-413 and DLG-1 in the polarization of the spermathecal cells. The spermatheca forms an accordion-like organ through which eggs must enter to complete the ovulation process. LET-413- and DLG-1-depleted animals exhibit failure of ovulation. Consistent with this phenotype, the assembly of the apical junction into a continuous belt fails and the PAR-3 protein and microfilaments are no longer localized asymmetrically. All these defects can be suppressed by mutations in IPP-5, an inositol polyphosphate 5-phosphatase and in ITR-1, an inositol triphosphate receptor, which both are supposed to increase the intracellular Ca2+ level. Analysis of embryogenesis revealed that IP3/Ca2+ signaling is also required during junction assembly in embryonic epithelia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 327, Issue 1, 1 March 2009, Pages 34-47
Journal: Developmental Biology - Volume 327, Issue 1, 1 March 2009, Pages 34-47
نویسندگان
Jennifer Pilipiuk, Christophe Lefebvre, Tobias Wiesenfahrt, Renaud Legouis, Olaf Bossinger,