کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10963939 | 1102697 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthetic virus seeds for improved vaccine safety: Genetic reconstruction of poliovirus seeds for a PER.C6® cell based inactivated poliovirus vaccine
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
OPVIPVMVSTCID50Vaccine safety - ایمنی واکسنVaccine manufacturing - ساخت واکسنAdventitious agents - عوامل مخاطره آمیزInactivated poliovirus vaccine - واکسن ویروسی غیر فعال شدهoral poliovirus vaccine - واکسن پولیوویروس شفاهیPoliovirus vaccine - واکسن پولیویروسPoliovirus - پلیو ویروسTissue Culture Infectious Dose 50% - کشت بافت عفونی 50٪
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Safety of vaccines can be compromised by contamination with adventitious agents. One potential source of adventitious agents is a vaccine seed, typically derived from historic clinical isolates with poorly defined origins. Here we generated synthetic poliovirus seeds derived from chemically synthesized DNA plasmids encoding the sequence of wild-type poliovirus strains used in marketed inactivated poliovirus vaccines. The synthetic strains were phenotypically identical to wild-type polioviruses as shown by equivalent infectious titers in culture supernatant and antigenic content, even when infection cultures are scaled up to 10-25Â L bioreactors. Moreover, the synthetic seeds were genetically stable upon extended passaging on the PER.C6® cell culture platform. Use of synthetic seeds produced on the serum-free PER.C6® cell platform ensures a perfectly documented seed history and maximum control over starting materials. It provides an opportunity to maximize vaccine safety which increases the prospect of a vaccine end product that is free from adventitious agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 42, 13 October 2015, Pages 5498-5502
Journal: Vaccine - Volume 33, Issue 42, 13 October 2015, Pages 5498-5502
نویسندگان
Barbara P. Sanders, Diana Edo-Matas, Natasa Papic, Hanneke Schuitemaker, Jerome H.H.V. Custers,