کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10964254 | 1102708 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
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کلمات کلیدی
DTPAmAbsPRNFHADTPSPOT-synthesisMIPCPeptide array - آرایه پپتیدهpertussis toxin - سموم سورافنیFilamentous hemagglutinin - هموگلوتینین رشته ایDiphtheria–tetanus–pertussis vaccine - واکسن دیفتری- تانتان-کوسه ایMonoclonal antibodies - پادتنهای تَکتیرهPertactin - پرتاکتینSynthetic peptides - پپتیدهای مصنوعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine](/preview/png/10964254.png)
چکیده انگلیسی
Pertussis is a serious infectious disease of the respiratory tract caused by the gram-negative bacteria Bordetella pertussis. There has been a reemergence of this disease within the population of several countries that have well established vaccination programs. Analyzes of clinical isolates suggest an antigenic divergence between the vaccine-based strains to the circulating strains. Although antibodies against P.69 are involved in the observed protective immunity, the sequences recognized as antigenic determinants in P.133, the precursor for P.69, P.3.4 and P.30, have not be determined. Here, the precise mapping of linear B-cell epitopes within the predicted P.133 pertactin sequences was accomplished using the SPOT-synthesis of peptide arrays onto cellulose membranes and screening with murine sera generated by vaccination with either the Pertussis cellular (miPc) or Pertussis acellular (miPa) vaccine. A total of 23 major epitopes were identified by sera from miPc vaccinated mice, while thirteen were identified by sera from miPa vaccinated mice. Of these epitopes, 12 epitopes were specifically identified by antibodies produced in response to the miPc vaccine and two were specific to the miPa vaccine. These epitopes were distributed throughout the pertactin sequence but a significant number were concentrated to the P.30 Prn segment. An analysis of the epitope correlation homologies indicated that the variations from the observed mutations in pertactin would not constitute a problem using these vaccines. In addition, the mapping of epitopes demonstrated a higher number of linear B-cell epitopes immunized with the Pc vaccine than the Pa vaccine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 47, 29 October 2014, Pages 6251-6258
Journal: Vaccine - Volume 32, Issue 47, 29 October 2014, Pages 6251-6258
نویسندگان
Flavio R. da Silva, Paloma Napoleão-Pego, Salvatore G. De-Simone,