کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10964331 | 1102710 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Maternal immunization with chimpanzee adenovirus expressing RSV fusion protein protects against neonatal RSV pulmonary infection
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کلمات کلیدی
qRT-PCRRSVAd5FI-RSVHuman adenovirus serotype 5RSV vaccineAdenovirus - آدنوویروسMaternal immunization - ایمن سازی مادرانQuantitative reverse-transcriptase polymerase chain reaction - واکنش زنجیره ای پلیمراز معکوس ترانس کریتازRespiratory syncytial virus - ویروس سنسیتیال تنفسیF protein - پروتئین FFusion protein - پروتئین فیوژن یا پروتئین همجوشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease with high morbidity and mortality in young infants and children. Despite numerous efforts, a licensed vaccine against RSV remains elusive. Since young infants form the primary target group of RSV disease, maternal immunization to boost the protection in neonates is an attractive strategy. In this study we tested the efficacy of maternal immunization with a chimpanzee adenovirus expressing codon-optimized RSV fusion protein (AdC7-Fsyn) to protect infants against RSV infection. Single intranasal immunization of mice by AdC7-Fsyn induced robust anti-RSV systemic and mucosal immunity that protected against RSV without causing vaccine-enhanced RSV disease. RSV humoral immunity was transferred to pups born to immunized mothers that provided protection against RSV. Immunization with AdC7-Fsyn was effective even in the presence of Ad5 preimmunity. The maternally derived immunity was durable with the half-life of 14.63 days that reduced the viral replication up to 15 weeks of age. Notably, the passively immunized mice could be actively re-immunized with AdC7-Fsyn to boost and extend the protection. This substantiates maternal immunization with an AdC7-based vaccine expressing RSV F as feasible approach to protect against RSV early in life.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 43, 29 September 2014, Pages 5761-5768
Journal: Vaccine - Volume 32, Issue 43, 29 September 2014, Pages 5761-5768
نویسندگان
Anurag Sharma, Rebecca Wendland, Biin Sung, Wenzhu Wu, Thomas Grunwald, Stefan Worgall,