کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10964707 | 1102724 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A gold glyco-nanoparticle carrying a listeriolysin O peptide and formulated with Advax⢠delta inulin adjuvant induces robust T-cell protection against listeria infection
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ADVAXCFUi.vGold glyconanoparticlesLLOGNPCTSDMIICAPCi.pantigen-presenting cells - آنتیژن ارائه سلولintra-peritoneal - داخل صفاقیintra-venous - داخل وریدیDendritic cells - سلول های دندریتیکListeria monocytogenes - لیستریا مونوسیتوژنزListeriosis - لیستریوزیسListeriolysin O - لیستریولیزین OMacrophages - ماکروفاژها،درشت خوارهاmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیMHC - مجموعه سازگاری بافتی اصلیcolony forming units - واحدهای تشکیل دهنده کلنیVaccines - واکسنMø - وزارت معارفCathepsin-D - کاتهپسین-DGlyconanoparticles - گلیکانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the search for an effective vaccine against the human pathogen, Listeria monocytogenes (Listeria), gold glyconanoparticles (GNP) loaded with a listeriolysin O peptide LLO91-99 (GNP-LLO) were used to immunise mice, initially using a dendritic cell (DC) vaccine approach, but subsequently using a standard parenteral immunisation approach. To enhance vaccine immunogenicity a novel polysaccharide adjuvant based on delta inulin (Advaxâ¢) was also co-formulated with the GNP vaccine. Confirming previous results, DC loaded in vitro with GNP-LLO provided better protection against listeriosis than DC loaded in vitro using free LLO peptide. The immunogenicity of GNP-LLO loaded DC vaccines was further increased by addition of Advax⢠adjuvant. However, as DC vaccines are expensive and impracticable for prophylactic use, we next asked whether the same GNP-LLO antigen could be used to directly target DC in vivo. Immunisation of mice with GNP-LLO plus Advax⢠adjuvant induced LLO-specific T-cell immunity and protection against Listeria challenge. Protection correlated with an increased frequency of splenic CD4+ and CD8+ T cells, NK cells and CD8α+ DC, and Th1 cytokine production (IL-12, IFN-γ, TNF-α, and MCP-1), post-challenge. Enhanced T-cell epitope recruitment post-challenge was seen in the groups that received Advax⢠adjuvant. Immunisation with GNP-LLO91-99 plus Advax⢠adjuvant provided equally robust Listeria protection as the best DC vaccine strategy but without the complexity and cost, making this a highly promising strategy for development of a prophylactic vaccine against listeriosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 12, 17 March 2015, Pages 1465-1473
Journal: Vaccine - Volume 33, Issue 12, 17 March 2015, Pages 1465-1473
نویسندگان
Estela Rodriguez-Del Rio, Marco Marradi, Ricardo Calderon-Gonzalez, Elisabet Frande-Cabanes, Soledad Penadés, Nikolai Petrovsky, Carmen Alvarez-Dominguez,