کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10965819 | 1102760 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preclinical immunogenicity and functional activity studies of an AÂ +Â W meningococcal outer membrane vesicle (OMV) vaccine and comparisons with existing meningococcal conjugate- and polysaccharide vaccines
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کلمات کلیدی
OPASBAOMVMeningitis beltAfrica - افریقاMeningococcal disease - بیماری مننگوکوکOuter membrane vesicle - غشای بیرونی vesicleOpsonophagocytic activity - فعالیت OpsonophagocyticSerum bactericidal activity - فعالیت ضد باکتری سرمNeisseria meningitidis - نایسریا مننژیتیس، مننگوکوکVaccine - واکسنOuter membrane protein - پروتئین غشای خارجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preclinical immunogenicity and functional activity studies of an AÂ +Â W meningococcal outer membrane vesicle (OMV) vaccine and comparisons with existing meningococcal conjugate- and polysaccharide vaccines Preclinical immunogenicity and functional activity studies of an AÂ +Â W meningococcal outer membrane vesicle (OMV) vaccine and comparisons with existing meningococcal conjugate- and polysaccharide vaccines](/preview/png/10965819.png)
چکیده انگلیسی
Meningococci of serogroups A and W (MenA and MenW) are the main causes of epidemic bacterial meningitis outbreaks in sub-Saharan Africa. In this study we prepared a detergent extracted outer membrane vesicle (dOMV) vaccine from representative African MenA and MenW strains, and compared the immunogenicity of this vaccine with existing meningococcal conjugate and polysaccharide (PS) vaccines in mice. NMRI mice were immunized with preclinical batches of the AÂ +Â W dOMV vaccine, or with commercially available vaccines; a MenA conjugate vaccine (MenAfriVac®, Serum Institute of India), ACYW conjugate vaccine (Menveo®, Novartis) or ACYW PS vaccine (Mencevax®, GlaxoSmithKline). The mice received 2 doses of 1/10 or 1/50 of a human dose with a three week interval. Immune responses were tested in ELISA, serum bactericidal activity (SBA) and opsonophagocytic activity (OPA) assays. High levels of IgG antibodies against both A and W dOMV were detected in mice receiving the AÂ +Â W dOMV vaccine. High SBA titers against both MenA and MenW vaccine strains were detected after only one dose of the AÂ +Â W dOMV vaccine, and the titers were further increased after the second dose. The SBA and OPA titers in mice immunized with dOMV vaccine were significantly higher than in mice immunized with the ACYW-conjugate vaccine or the PS vaccine. Furthermore, the AÂ +Â W dOMV vaccine was shown to induce SBA and OPA titers against MenA of the same magnitude as the titers induced by the A-conjugate vaccine. In conclusion, the AÂ +Â W dOMV vaccine induced high levels of functional antibodies to both MenA and MenW strains, levels that were shown to be higher or equal to the levels induced by licensed meningococcal vaccines. Thus, an AÂ +Â W dOMV vaccine could potentially serve as an alternative or a supplement to existing conjugate and PS vaccines in the African meningitis belt.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 51, 9 December 2013, Pages 6097-6106
Journal: Vaccine - Volume 31, Issue 51, 9 December 2013, Pages 6097-6106
نویسندگان
G. Tunheim, M. Arnemo, L.M. Næss, Ã
.K. Fjeldheim, L. Nome, K. Bolstad, A. Aase, A. Mandiarote, H. González, D. González, L. GarcÃa, D. Cardoso, G. Norheim, E. Rosenqvist,