Immunisation of mice with Mycoplasma hyopneumoniae antigens P37, P42, P46 and P95 delivered as recombinant subunit or DNA vaccines

► The search for novel vaccines against porcine enzootic pneumonia is mandatory. ► We evaluated mice immunisation with P37, P42, P46 and P95 Mycoplasma hyopneumoniae antigens. ► P42 and P95 recombinant subunit vaccines induced strong immune response. ► P46 DNA vaccine induced immune response similar to P42 and P95 subunit vaccines. ► All vaccines induced upregulation of INFγ and downregulation of IL1 and TNFα.