Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins

► The 10 putative OMPs were cloned, expressed and purified as HIS fusion proteins. ► The rGAPDH, rOapA, and rHPS-0675 individually could induce good protective in mice. ► Three proteins elicited good immune response in alone or combination in piglets. ► Combination of three proteins offered best protection against H. parasuis in piglets. ► The rGAPDH, rOapA, and rHPS-0675 can be a promising subunit vaccine candidate.