Prime-boost BCG vaccination with DNA vaccines based in β-defensin-2 and mycobacterial antigens ESAT6 or Ag85B improve protection in a tuberculosis experimental model

► Prime-boost BCG with DNA vaccines improved effectiveness against BCG alone. ► DNA vaccine with gene beta-defensin-2 increases the antigenic proprieties of ESAT6. ► DNA vaccine with gene beta-defensin-2 increases the antigenic proprieties of Ag85B.