Delivery of antigenic candidates by a DNA/MVA heterologous approach elicits effector CD8+T cell mediated immunity against Trypanosoma cruzi

► DNA-rMVAs-encoding TcG2/TcG4 primed type 1 immunity expanded robustly upon Trypanosoma cruzi infection. ► Immunization with TcG2/TcG4 provided resistance to T. cruzi and chronic myocarditis. ► TcG2 and TcG4 are excellent candidates for vaccine against Chagas disease.