Evaluation of protective effect of DNA vaccines encoding the BAB1_0263 and BAB1_0278 open reading frames of Brucella abortus in BALB/c mice

► DNA vaccines pVF263 and pVF278, stimulate a Th1-cell-mediated immune responses. ► DNA vaccines pVF263 and pVF278, stimulate antibody production. ► DNA plasmid pVF278 induces a better protection in mice than pVF263. ► This study provides the first evidence with protective effect for BAB1_0278 antigen.