Recombinant outer membrane secretin PilQ406-770 as a vaccine candidate for serogroup B Neisseria meningitidis

► We overexpressed recombinant PilQ406-770 with IPTG and affinity-purified by Ni-NTA agarose. ► We evaluate vaccine potential of recombinant PilQ406-770 using active immunization. ► Increasing serum IgG responses in immunized group with PilQ406-770. ► Increasing serum bactericidal activity against serogroups A and B in immunized group with PilQ406-770.