Recombinant canine distemper virus serves as bivalent live vaccine against rabies and canine distemper

► We developed a promising bivalent live vaccine against rabies and canine distemper. ► This vaccine was safe in mice and dogs. ► Vaccination of this vaccine completely protected mice from RABV street virus challenge. ► This vaccine induced a strong neutralizing antibody response to RABV and CDV in dogs.