Wide-range protection against avian reovirus conferred by vaccination with representatives of four defined genotypes

► Avian reovirus has been classified into four genotypes. ► A vaccine was developed, comprised of four representatives, one from each genotype. ► Vaccination with a representative protected against isolates of its genotype. ► Mix of representatives conferred protection against field isolates of all genotypes. ► The 4-valent vaccine induces broad protection against avian reovirus.