Enhancement of immune response to a DNA vaccine against Mycobacterium tuberculosis Ag85B by incorporation of an autophagy inducing system

► Kinase defective mTOR induces autophagy. ► Chitosan nanoparticles effectively delivers DNA vaccine containing Mycobacterium tuberculosis Ag85B and autophagy inducing plasmid. ► DNA vaccine incorporating kinase defective mTOR increases Th1 immune response against Ag85B.