High-throughput proteomic screening identifies Chlamydia trachomatis antigens that are capable of eliciting T cell and antibody responses that provide protection against vaginal challenge

► Chlamydia proteomic screen yielded T cell antigens for vaccine development. ► Screening of peripheral T cells can identify T cell antigens regardless of MHC allelic expression. ► Antigens stimulate polyfunctional CD4+ T cell and antibody responses. ► Vaccination provides long-lived protection against challenge.