Ascaris suum enolase is a potential vaccine candidate against ascariasis

► The present study cloned the gene encoding Ascaris suum enolase (As-enol-1). ► As-enol-1 was highly conserved between different nematodes. ► pVAX-Enol induced a high level of humoral and cellular responses against A. suum. ► pVAX-Enol induced a 61.4% reduction in larvae recovery compared with blank control. ► A. suum enolase is a potential vaccine candidate against A. suum infection.