Immunogenicity of a lentiviral-based DNA vaccine driven by the 5′LTR of the naturally attenuated caprine arthritis encephalitis virus (CAEV) in mice and macaques

► We used the LTR of the attenuated goat CAEV to express HIV genes in a DNA vaccine. ► This vaccine expresses efficiently all driven genes in transfected cells. ► This vaccine induces potent T cell responses in mice and macaques. ► This vaccine is safer since this LTR does not integrate with HIV integrase.